Apr 1, 2024 · To restore the anaerobic environment, the bioreactors were flushed with pure N 2 before sealing, except the reactors for Batch#3, which were flushed with pure H 2.At regular time intervals (t = 0 h; 24 h; 48 h; 72 h; 144 h; 192 h), the reactors were sampled to perform the chemical characterization of the fermentation broth.